Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Activation of IP 3 R in atrial cardiomyocytes leads to generation of cytosolic cAMP

doi: 10.1152/ajpheart.00152.2024

Figure Lengend Snippet: Inositol trisphosphate (IP 3 ) pathway is active in human atrial induced pluripotent stem cells (iPSCs) as shown using multielectrode array. A : representative averaged multielectrode array (MEA) field action potential waveforms from control (dimethylsulfoxide, DMSO; n = 7), phenylephrine (PE; 30 μM; n = 11), and PE (1 or 30 μM) in the presence of either H89 (1 μM; n = 7) or MDL-12330A (3 μM; n = 1–10). B : effect of H89 (1 μM) and MDL-12330A (3 μM) on the beat rate of human iPSCs-derived atrial cardiomyocytes (hiPSC-ACMs) in response to PE at concentrations of 1–30 μM (DMSO, n = 7; H89, n = 7; MDL-12330A, n = 10). Data are represented as means ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001, 2-way repeated-measures ANOVA followed by Sidak’s multiple comparisons. IP 3 , inositol (1,4,5)-trisphosphate.

Article Snippet: For multielectrode array (MEA) experiments, human induced pluripotent stem cells-derived atrial cardiomyocytes (hiPSC-ACMs) (ax2518, Axol Bioscience, UK) were cultured at 5.0 × 10 5 cells/well on a 768-channel 48-well Axion Cytoview MEA 48 plate (M768-tMEA-48B, Axion Biosystems, USA) coated with Axol’s Fibronectin Coating Solution (ax0049, Axol Bioscience, UK) at 37°C in a 5% CO 2 –95% air atmosphere.

Techniques: Control, Derivative Assay

Journal: bioRxiv

Article Title: Cardiac defects in spinal muscular atrophy and the role of SMN in cardiomyocyte homeostasis

doi: 10.64898/2026.03.20.713246

Figure Lengend Snippet: KO schematic in cardiomyocytes B-D: Oxygen consumption rate (OCR) is significantly increased in siSMN-treated cardiomyocytes compared with siScramble controls and extracellular acidification rate (ECAR) is significantly decreased in siSMN-treated cardiomyocytes. n = 16 technical replicates. * p <0.05. **p < 0.01. Mitochondrial and glycolytic ATP production rates show no significant difference between siSMN and siScramble conditions (ns). E: Volcano plot of differential gene expression following SMN knockdown. F: Heatmap and hierarchical clustering of differentially expressed genes demonstrate distinct transcriptional profiles between siSMN and siScramble cardiomyocytes. G: Pathway enrichment analysis of differentially expressed genes identifies significant perturbation of multiple signaling pathways, including enrichment of PTEN signaling.

Article Snippet: Human cardiomyocytes (Axol Bioscience Limited, Cambridgeshire, England; Cat. No. ax2520; Lot No. 2520310317) were cultured and differentiated in coated plates using supplemented media from the Human iPSC-Derived Ventricular Cardiomyocyte Kit (Axol Bioscience Limited, Cambridgeshire, England).

Techniques: Gene Expression, Knockdown, Protein-Protein interactions

Journal: bioRxiv

Article Title: Cardiac defects in spinal muscular atrophy and the role of SMN in cardiomyocyte homeostasis

doi: 10.64898/2026.03.20.713246

Figure Lengend Snippet: Ingenuity pathway analysis of differentially expressed genes after SMN2 knockdown in human cardiomyocytes, showing the top 20 significantly enriched canonical pathways ranked by –log(p value). Bar color denotes predicted directionality based on z-score: black indicates negative z-score (predicted pathway inhibition), red indicates positive z-score (predicted pathway activation), and gray indicates no consistent activation pattern.

Article Snippet: Human cardiomyocytes (Axol Bioscience Limited, Cambridgeshire, England; Cat. No. ax2520; Lot No. 2520310317) were cultured and differentiated in coated plates using supplemented media from the Human iPSC-Derived Ventricular Cardiomyocyte Kit (Axol Bioscience Limited, Cambridgeshire, England).

Techniques: Knockdown, Inhibition, Activation Assay